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SYSTAT exponential curve fit
mTAP-CA1 synapses and putative population spikes exhibit an enduring form of long-term potentiation (LTP) in freely moving rats. (A) Daily mTAP-CA1 (•, n = 6) and cCA3-CA1 (°, n = 6) slope analysis. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. Three out of six animals exhibited heterosynaptic long-term depression following TBS of the medial TAP. (B) Curve fitting of post LTP daily slope averages indicate mTAP-CA1 LTP decay ( n = 6) is represented as a sum of two <t>exponential</t> curves with time constants (τ) of 2.7 days and 148 days to decay 63.2% of maximal LTP. (C) Daily mTAP-CA1 putative population spike amplitude analysis in animals that exhibited population spike potentiation following TBS ( n = 4). Significant differences between post- and pre-tetanus putative population spike amplitudes persist for 5 days after LTP induction [* p < 0.05, repeated measures one way ANOVA]. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. (D) Curve fitting of post LTP daily mTAP-CA1 putative population spike amplitude averages indicates population spike potentiation decays exponentially with a time constant (τ) of 9.6 days to decay 63.2% of maximal LTP.
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mTAP-CA1 synapses and putative population spikes exhibit an enduring form of long-term potentiation (LTP) in freely moving rats. (A) Daily mTAP-CA1 (•, n = 6) and cCA3-CA1 (°, n = 6) slope analysis. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. Three out of six animals exhibited heterosynaptic long-term depression following TBS of the medial TAP. (B) Curve fitting of post LTP daily slope averages indicate mTAP-CA1 LTP decay ( n = 6) is represented as a sum of two exponential curves with time constants (τ) of 2.7 days and 148 days to decay 63.2% of maximal LTP. (C) Daily mTAP-CA1 putative population spike amplitude analysis in animals that exhibited population spike potentiation following TBS ( n = 4). Significant differences between post- and pre-tetanus putative population spike amplitudes persist for 5 days after LTP induction [* p < 0.05, repeated measures one way ANOVA]. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. (D) Curve fitting of post LTP daily mTAP-CA1 putative population spike amplitude averages indicates population spike potentiation decays exponentially with a time constant (τ) of 9.6 days to decay 63.2% of maximal LTP.

Journal: Frontiers in Neural Circuits

Article Title: Long-term Potentiation at Temporoammonic Path-CA1 Synapses in Freely Moving Rats

doi: 10.3389/fncir.2016.00002

Figure Lengend Snippet: mTAP-CA1 synapses and putative population spikes exhibit an enduring form of long-term potentiation (LTP) in freely moving rats. (A) Daily mTAP-CA1 (•, n = 6) and cCA3-CA1 (°, n = 6) slope analysis. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. Three out of six animals exhibited heterosynaptic long-term depression following TBS of the medial TAP. (B) Curve fitting of post LTP daily slope averages indicate mTAP-CA1 LTP decay ( n = 6) is represented as a sum of two exponential curves with time constants (τ) of 2.7 days and 148 days to decay 63.2% of maximal LTP. (C) Daily mTAP-CA1 putative population spike amplitude analysis in animals that exhibited population spike potentiation following TBS ( n = 4). Significant differences between post- and pre-tetanus putative population spike amplitudes persist for 5 days after LTP induction [* p < 0.05, repeated measures one way ANOVA]. Inset: baseline (solid line) and post-TBS (dashed line) mTAP-CA1 responses. Calibration: 0.5 mV, 5 ms. (D) Curve fitting of post LTP daily mTAP-CA1 putative population spike amplitude averages indicates population spike potentiation decays exponentially with a time constant (τ) of 9.6 days to decay 63.2% of maximal LTP.

Article Snippet: Averaged daily data were plotted with an exponential curve fit (SigmaPlot 12, Systat Software, San Jose, CA, USA) to determine the LTP decay time constant (τ), defined as the time of decaying 63.2% of maximal LTP.

Techniques: